The reference intakesStrong evidence
The UK RNI is 0.75 g per kg a day, set by the Department of Health in 1991. The US RDA is 0.8 g per kg "for the reference body weight", from an estimated average requirement of 0.66. WHO's safe level of 0.83 g per kg is "sufficient for 97.5% of the population" and "the same for both sexes, at all ages, and for all body weights within the acceptable range", and EFSA uses the same 0.83, including for older adults.
Sources 1, 2, 22, 3, 4
For muscle, the benefit levels off at about 1.6 g per kgStrong evidence
In 49 trials with 1,863 people, protein supplements added 0.30 kg of fat-free mass and 2.49 kg to the one-rep max, and gains levelled off at 1.62 g per kg a day (95% confidence interval 1.03 to 2.20). The breakpoint wasn't statistically significant (p = 0.079), and because its confidence interval reached 2.20, the authors suggest "~2.2 g protein/kg/d for those seeking to maximise resistance training-induced gains". In 74 trials, more protein added a little lean mass with strength training, significantly at 1.2 to 1.59 g per kg in people 65 and over and at 1.6 or more in younger people. In 105 articles, each extra 0.1 g per kg added 0.39 kg of lean mass below 1.3 g per kg and 0.12 kg above it.
Sources 5, 16, 23
Sports nutrition advice runs higherEmerging evidence
The International Society of Sports Nutrition (ISSN) says 1.4 to 2.0 g per kg a day "is sufficient for most exercising individuals", and that 2.3 to 3.1 g may help lean, trained people keep muscle while eating less; several of its authors declare industry funding or consultancy. The British Nutrition Foundation reports other sports bodies' ranges of 1.2 to 2.0 g, and says higher intakes "would not be recommended for the general population".
Sources 15, 1
Whether older adults need moreMixed evidence
Expert groups, PROT-AGE and the European Society for Clinical Nutrition and Metabolism (ESPEN), advise at least 1.0 to 1.2 g per kg a day from 65. Trials found more lean mass with strength training at 1.2 to 1.59 g in people 65 and over, and better lean mass with higher protein during weight loss. But WHO found "no firm evidence warranting different values" for older people, and EFSA keeps one value for all adults.
Sources 6, 7, 16, 24, 3, 4
More protein keeps more lean mass while losing weightStrong evidence
In 24 trials (1,063 people, about 12 weeks), energy-restricted higher-protein diets lost 0.79 kg more weight and 0.87 kg more fat, and 0.43 kg less fat-free mass, than standard-protein diets. In 20 trials in older adults, people on higher-protein diets "retained more lean mass and lost more fat mass during weight loss".
Sources 8, 24
How much, when losing weightEmerging evidence
A review suggests diets with "between 1.2 and 1.6 g protein" per kg a day. A 2025 advisory for people on glucagon-like peptide-1 (GLP-1) medicines, such as semaglutide and tirzepatide, notes the same range "proposed during active weight reduction", and adds that more protein alone "is likely inadequate" to preserve muscle without strength training. In 40 young men eating 40% less and training hard 6 days a week, 2.4 g per kg gained 1.2 kg of lean mass in 4 weeks, against 0.1 kg at 1.2 g.
Sources 9, 10, 25
Per meal, there's no fixed limitEmerging evidence
In single doses of up to 40 g, muscle protein building levelled off after about 0.24 g per kg in young men and 0.40 g in older men. But 40 g beat 20 g after whole-body training, and 100 g gave "a greater and more prolonged (>12 h) anabolic response" than 25 g. Over 12 hours, four 20 g doses beat eight of 10 g and two of 40 g.
Sources 26, 27, 12, 28
Healthy kidneys cope with more proteinStrong evidence
In 28 trials of 1,358 adults without kidney disease, higher protein (1.5 g per kg or more, 20% of energy or more, or 100 g a day or more) didn't change kidney function. WHO says claims of kidney damage in otherwise healthy people don't "stand up to scrutiny".
Sources 14, 3
Reduced kidney function is differentEmerging evidence
In 1,624 women followed for 11 years, protein wasn't linked with decline when kidney function was normal, but with mildly reduced function each extra 10 g a day went with a faster fall, more so for non-dairy animal protein. KDIGO suggests avoiding more than 1.3 g per kg in CKD at risk of getting worse.
Sources 29, 13
No upper limit has been setStrong evidence
The US and Canada list the upper limit as not determined. EFSA says the data "are not sufficient" to set one and that twice its reference intake "is considered safe", and WHO says twice its safe level is unlikely to carry any risk. In 1991 the UK advised adults to avoid more than twice the RNI.
Sources 17, 4, 3, 1
Plant protein builds muscle tooEmerging evidence
In 38 young men training for 12 weeks, a vegan diet with soy protein at 1.6 g per kg matched a mixed diet with whey for muscle and strength gains. In the UK, intakes are above the reference intake on average, "including in vegetarians and vegans".
Sources 30, 1